Changes in plasmodesmatal permeability of the staminal hairs of Setcreasea purpurea during development
Yang Shijie, Li Mingyi, Zhang Xiaoying
Plant Physiology Laboratory, Beijing Agricultural University. Beijing 100094
Abstract
A range of water-soluble fluorescent dyes of known molecular weights were injected into staminal hairs of Setcreasea purpurea at different developmental stages by pressure with Opton Microinjection System. Fluorescein isothiocyanate (FITC) was conjugated to (Ala)6 and Leu-(Arg)2-Ala -Ser-Val-Ala. and purified. LRB and FITC-dextran were made up as 1mmol/L solution, and the movement of the fluorecein probes was observed under the fluorescence microscope (Olympus RFL) 5min after the beginning of dye injection. Only where dyes was seen in the neighboring cells was it cored as dye movement. The results show that plasmodesmatal permeability changes greatly with the development of staminal hairs. the molecular exclusion limit of plasmodemata in bud IV is less than 1000D which is similar to that found in previous works.
Key words: Plasmodesmata;Microinjection;Intercellular transport;Setcreasea purpurea
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Citing This Article:
Yang Shijie, Li Mingyi, Zhang Xiaoying. Changes in plasmodesmatal permeability of the staminal hairs of Setcreasea purpurea during development. Acta Physiol Sin 1995; 47 (4): (in Chinese with English abstract).