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Effect of epi-BR on activity of peroxidase and soluble protein content of cucumber cotyledon

Ding Wenming, Zhao Yuju

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

It is shown that epi-brassinolide (epi-BR, 0.5mg/L), a new synthetic brassinolide, significantly reduced the content of chlorophyll a, chlorophyll b in cucumber cotyledons and the ratio of chlorophyll a/ chlorophyll b. Further studies showed that in the cotyledons epi-Br increased the activity of peroxidase, which is involved in the breakdown of the chlorophyll. In addition, epi-BR de-creased the content of soluble protein in cucumber cotyledons (mainly the RuBPcase) and caused the accumulatin of free aminc acids. All of these effects of epi-BR on cucumber cotyledons indicates that epi-BR accelerates the senescence of cucumber cotyledons.

Key words: Epi-brassinolide;Cucumber cotyledons;Peroxidase;Chlorophyll;Soluble protein;Free amino acids

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Citing This Article：

Ding Wenming, Zhao Yuju. Effect of epi-BR on activity of peroxidase and soluble protein content of cucumber cotyledon. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).