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Changes of translatable mRNA in wheat primary leaves at the initial stage of stripe rust infection

Shang Hongsheng, Zhang Hui, Li Zhenqi

Department of Plant Protection, Northwesten Agricultural University. Yangling 712100,Shaanxi

Abstract

At the initial stage of infection of wheat (Triticumm aestivum) L. cv. Lovrin No13) by a virulent race (CY29) and its avirulent mutant (CY 29-mut3) of stripe rust (Puccinia striiformis) west), a rapid increase in translatable mRNA level in host leaves of incompatible combination and a delayed increase in host leaves of compatible combination were observed. Meanwhile, the former showed a higher level of Poly (A~(+)_-RNA and the later was lower than uninoculated control. ~(32)P in vivo) labelling experiment confirmed that the increase of Poly )A~(+)-RNA synthesis in host leaves of incompatible combination occurred earlier than that in the compatible combination. Autoradiography of one-dimensional SDS-PAGE seperation of Poly (A~(+)-RNA in vitro) translation products indicated that there were quantitative differences in the incorporation of ~(35)S-Met in polypeptides.

Key words: Wheat stripe rust;Incompetibility and competibility;In vitro translation;Poly [A~(+)]-RNA;RNA synthesis

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Citing This Article：

Shang Hongsheng, Zhang Hui, Li Zhenqi. Changes of translatable mRNA in wheat primary leaves at the initial stage of stripe rust infection. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).