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Mechanism of direct redifferentiation of flower bud from explant of Dianthus chinensis L. by cytokinin inducement

Zhou Youyuan, Wu Zunbiao, Liu Youqi, Yang Yi

Department of Biology, Hubei University. Wuhan 430062,Hubei

Abstract

Using the MS+2,4-D 0.1mg/L as the basic culture medium to which different concentration of zeation, benzylaminopurine and kinetin were added. Direct differentiation of blade, stem and calys explant to flower bud was studied. The results show that the kind and dosage of hormone or growth regulating substance are dicisive factors in inducing flower differentiation. But the response to hormone or growth regulating substance is different for different parts of explants. On the other hand, if the blooing condition is given, the callus can by pass through the vegetative growing stage and form directly the reproductive stage and differentiate to form flower bud with no leaf.

Key words: Explant;Cytokinin;Zeatin;Benzylaminopurine;Kinetin;Callus tissue

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Citing This Article：

Zhou Youyuan, Wu Zunbiao, Liu Youqi, Yang Yi. Mechanism of direct redifferentiation of flower bud from explant of Dianthus chinensis L. by cytokinin inducement. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).