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Ti plasmid-mediated transformation of tobacco with C_(4)-PEPCase cDNA from Zea mays

Dong Longying, Ling Jun, Wang Chaoming, Shi Jiaonai

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

A2.0kb fragement of C_(4)-PEP-Case cDNA derived from a maize PEP-Case cDNA clone pPEP3055 was subcloned into binary vector pBin 19, and transconjugated into Agrobacterium tume-faciens) retaining vir helper plasmid LBA 4404 by means of ‘triparental mating’. The the engineering plasmid pBPC2 harbouring C_(4)-PEPCase cDNA was transformed into the leaf disc of tobacco by co-culture with transconiugated A. tumefaciens). Antibiotic screening yielded a batch of transformants resistant to kanamycin and the regenerated transgenic tobacco was obtained.

Key words: Agrobacterium tumefaciens;Ti plasmid;PEPCase cDNA;Tobacco;Gene transformation

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Citing This Article：

Dong Longying, Ling Jun, Wang Chaoming, Shi Jiaonai. Ti plasmid-mediated transformation of tobacco with C_(4)-PEPCase cDNA from Zea mays. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).