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Over-expression of maize chloroplast atp B and atp E genes in Escherichia coli

Wang Zhenguo, Wei Jiamian, Shen Yungang

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

We constructed expressioin plasmids for high-level expression in Escherichia coli) of entire #beta# subunit of CF_(1) of maize chloroplast, #beta# subunit from which had been deleted twenty-three amino acid residues in the N-terminal region and entire #epsilon# subunit. SDS-polyacrylamide gel electrophoresis and Western blot experiments showed that the degree of expression of these proteins in E. coli) cells containing the corresponding expression plasmids reached saturuation after induction for 3h, accounting for 10% of total cell proteins respectively These expression products formed insoluble inclusion bodies in E. coli) cells and can be solubilized in 5mol/L urea after fractionation of E. coli) cell proteinase K.

Key words: Dsoroplast;atpB gene;atpE gage

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Citing This Article：

Wang Zhenguo, Wei Jiamian, Shen Yungang. Over-expression of maize chloroplast atp B and atp E genes in Escherichia coli. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).