Issue Archive

Properties of maize chloroplast atpB gene expression products and effects of its antibody on the functions of CF_(1)

Wang Zhenguo, Wei Jiamian, Shen Yungang

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

Maize chloroplast atpB fusion and non-fusion genes were over-expressed in Escherichia coli. These expression products in E. coli were partially purified. They all exhibited very weak ATP-hydrolyzing activities. Anti-serum against fusion protein #beta#_(1) had no obvious effects on the photophosphorylation of spinach chloroplasts and Ca~(2+)-ATPase activity of soluble CF_(1) (Tables 4,5). However, it could increase the activity of soluble CF_(1) Mg~(2+)-ATPase stimulated by methanol. This indicates that soluble CF_(1) stimulated by methanol may show both activies of Mg~(2+)-and Ca~(2+)-ATPase, but their conformations at hydrolyzing ATP may be different.

Key words: Chloroplast;atpB gene expression products antiserum asainst;#beta# subunit;Photophosphorylation;ATPase activity

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Wang Zhenguo, Wei Jiamian, Shen Yungang. Properties of maize chloroplast atpB gene expression products and effects of its antibody on the functions of CF_(1). Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).