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Some biochemical characteristics of rice somatic and zygotic embryos

Xu Zhuyun, Wang Tongyu, He Meiying, Tang Xihua

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

an analysis of protein extracts by SDS--polymacrylamide gel electrophoresis showed that the protein composition was simliar in zygotic embryos (ZE) and the somatic embryos (SE) of rice cultured in vitro. About 17 bands were identical in the two embryo types according to their molecular weights. Among these bands, 67---94 kD polypeptides were present in much lower amounts in nenembryogenic calli (NE), and 39, 47 and 60 kD polypeptides were absent from NE. The amino acid components of free amino acid pool were also similar in ZE (7 d after anthesis) and SE. The predominant amino acids were Asp, Glu, Ala and Lys, and the Arg content increased in both embryos types during a later stage of differentiation, whereas the contents of these amino acids were lower in NE. In addition, it was found that the lectin--like substance occurred in both embryo types, but no hemagglutination activity was found in extracts from NE without embryoids.

Key words: Rice;Somatic embryo;Zygotic embryo;Non-embryogenic callus;Protein;Amino acid;Hemagglutination activity

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Citing This Article：

Xu Zhuyun, Wang Tongyu, He Meiying, Tang Xihua. Some biochemical characteristics of rice somatic and zygotic embryos. Acta Physiol Sin 1995; 47 (1): (in Chinese with English abstract).