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Change of respiration of young sugarcane leaf tissue during protoplasts isolation and some factors influencing them

He Ruotian, Qin Wei

Research Centre, Guangxi Agricultural University. Nanning 530005, Guangxi

Abstract

Following a transient rise at the early stage, the respiratory rate of the tissue from young sugarcane leaf declined quickly during the enzymatic isolation of protoplasts. In the presence of the mixture of cell wall degrading enzymes, the respiratory rate of the young leaf tissues decreased with an increase of the concentration of mannitol used as osmoticum, but to a lesser extent in the absence of the cell wall degrading enzymes, The respiratory rate decreased with an increase of cellulase concentration when the concentrations of pectinase and mannitol were kept constant in the mixture. As compared with the pectinase, the harmful effect of cellulase onthe respiration of young sugarcane tissues is more powerful. The respiration of freshly prepared protoplasts from young sugarcane leaf was inactivated by the cell wall degradation products (CWDP), water--extract of cell wall, active and denatural by boil cell wall degrading enzyme mixture, and the strongest inactivation was produced by CWDP which inactivated the activities of the key respiratory enzymes, suggesting that the decrease of respiratory activity might be caused mainly by the toxicity of CWDP during the enzymatic isolation

Key words: Respiration;Cell wall degrading enzyme;Toxic factor;Protoplast;Sugarcane

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Citing This Article：

He Ruotian, Qin Wei. Change of respiration of young sugarcane leaf tissue during protoplasts isolation and some factors influencing them. Acta Physiol Sin 1995; 47 (1): (in Chinese with English abstract).