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Effects of long-term hypoxia of intracellular pH and membrane potential of cultured rat carotid body glomus cells

He Shufang

Shanghai Brain Research Institute, Chinese Academy of Sciences. Shanghai 200031

Abstract

Spraque-Dawley(SD) rats(weighing about 100 g) were kept in a decompression chamber for 7--10 days. Then the carotid bodies were removed from the anesthetized animals and dissociated to obtain individuals or small clusters of glomus cells. These cells were cultured under hypoxic condition(11% O_(2), 5% CO_(2), 84% N_(2))for 2--3days. Control samples from normal SD rats(normoxic rat)were cultured in normoxic(21% O_(2), 5% CO_(2), 74%N_(2))or hypoxic conditions. Intracellular pH(pHi) and membrane potential(MP)of glomus cells of both the groups were simultaneously measured with pH-sensitive and conventional microelectrodes. Long-term hypoxia decreased pHi and increased MP of the cultured glomus cells to a degree far greater than acute hypoxia did; The mean pHi and MP of the glomus cells of the normoxic rat, but cultured under hypoxic environment, recovered approximately to control values when measued under normoxic condition, whereas those of the long-term hypoxic rat cells did not.

Key words: Intracellular pH;membrane potential;Hypoxia;Carotid body;Glomus cell

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Citing This Article：

He Shufang. Effects of long-term hypoxia of intracellular pH and membrane potential of cultured rat carotid body glomus cells. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).