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A study on hypotensive mechanism of adrenomedullin (13-52)

Zhao Dong, Tian Qing, Zhao Yuntao, Gong Changning, Han Qide, Zhang Zhaokang, Tang Jian

Institute of Cardiovascular Research, Beijing Medical University. Beijing 100083；China

Abstract

In the present study the hypotensive mechanism of AdM (13--52) was investigated in rats, both in vitro and in vivo. It was found that the hypotensive effect of AdM (13--52) could be partially inhibited by L--N~(G)--nitro--arginine (LNNA), an inhibitor of nitric oxide synthase. The vasodilator effect of AdM (13--52) was dependent on vascular endothelium and inhibitor by LNNA in a dose--dependent manner. This LNNA induced inhibitory effect could be reversed with L--Arginine. In addition, the vasodilator effect of AdM (13--52) disappeared with methylene blue (MB), which blocked cGMP formation. Using radioimmunoassay it was shown that LNNA lowered, but AdM (13--52) elevated the vascular cGMP content, while vascular cGMP content was not altered by co--application of AdM (13--52) and LNNA. The above results suggest that the vasodilator effect of AdM (13--52) might be mediated by nitric oxide.

Key words: Adrenomedullin;Nitric oxide

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Citing This Article：

Zhao Dong, Tian Qing, Zhao Yuntao, Gong Changning, Han Qide, Zhang Zhaokang, Tang Jian. A study on hypotensive mechanism of adrenomedullin (13-52). Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).