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Effect of glucocorticoids and other steroids on arginine vasopressin release from rat hypothalamic slices

Liu Xiu, Wang Chunan, Ye Ali, Jiang Ping, Chen Yizhang

Department of Physiology, Second Military University. Shanghai 200433；China

Abstract

Rat hypothalamic slices sectioned with vibratone (containing paraventricular and supraoptic nuclei) were incubated in static microchamber and the Arginine vasopressin (AVP) released from the slices was measured by radioimmunoassay. The effect of glucocorticoids (GC) and other steroids on AVP release was investigated. The results were as 6 h (not includig the 90 min for recovery of slices). (2) Corticosterone (B) inhibited AVP release within 20 min in a dose-dependent manner from 10~(-7)to 10~(-4)mol/L. (3). Cortisol, 17 #beta#--estradiol, or testosterone (10~(-6)mol/L) also inhibited AVP release within 20 min, but dexamethasone, aldosterone, progesterone, RU 486, or cholesterol had no significant effect. (4)RU486 (10~(-5)--10~(-3)mol/L) could partially block the rapid inhibitory effect of corticosterone. These results suggested that GC might exert in situ a regulatory negative feedback action on the AVP release from hypothalamus of rat through a non--genomic rather than a genomic mechanism.

Key words: Hypothalamic slice;Aginine vasopressin;Glucocorticoids;Non-genomic

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Citing This Article：

Liu Xiu, Wang Chunan, Ye Ali, Jiang Ping, Chen Yizhang. Effect of glucocorticoids and other steroids on arginine vasopressin release from rat hypothalamic slices. Acta Physiol Sin 1995; 47 (2): (in Chinese with English abstract).