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Effects of fungal elicitor on shikonin derivatives fromation in Onosma paniculatum cell cultures

Ning Wen, Zhao Qunhua, Xia Zhonghao, Cao Riqiang

Department of Biology, Nanjing University. Nanjing,Jiangsu

Abstract

A crude elicitor preparation was extracted after autoclaving a 6-day-culture of Aspergillus sp. which could accelerate shikonin derivatives formation but irreversibly arrest cell growth in Onosma paniculatum cell cultures. The optimum elicitor concentration was found to be 600#mu#g carbohydrate/50 ml M_(9) medium. The Onosma paniculatum cells showed strongest response to the elicitor during the growth phase (8 d) which causing shikonin derivatives content a doubling of the control. The time of autoclaving (from 20 min to 2h)did not affect the elicitor^s activity. The fungal elicitor changed slightly the relative content of each of the shikonin derivatives.

Key words: Onosma Paniculatum;Fungal elicitor;Shikonin derivatives;Cell culture

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Citing This Article：

Ning Wen, Zhao Qunhua, Xia Zhonghao, Cao Riqiang. Effects of fungal elicitor on shikonin derivatives fromation in Onosma paniculatum cell cultures. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).