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The cDNA clones of chitin-binding-protein from rice seed endosperm

Yang Riyao, Wang Tongyu, Tang Xihua

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai

Abstract

Two cDNA clones of chitin-binding-protein from rice seed endosperm(ES-CBP): CRRY1 and CRRY2 were screened and isolated with the ES-CBP specific antibody from seed cDNA library(constructed in #lambda#gt-22A). The antiserum(selected after gene expression)can react with the purified ES-CBP. Furthermore, DNA of the positive positive clones were extracted and digested by EcoR I -Not I. Agarose-electrophoresis indicates that the size of the inserted fragments are 1.6kb and 1.0kb respectively.

Key words: ES-CBP;cDNA clone;Insert fragment;Antiserum screening

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Citing This Article：

Yang Riyao, Wang Tongyu, Tang Xihua. The cDNA clones of chitin-binding-protein from rice seed endosperm. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).