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Regulatory properties of cytosolic pyruvate kinase from young leaves of Passiflora edulis

Guo Zhenfei, Li Mingqi

Laboratory of Photosynthesis Research, South China Agricultural University. Guangzhou,Guangdong

Abstract

The PK_(c)from young leaves of P. edulis exhibited a Michaelis-Menten type kinetics for PEP and ADP, K_(m)(PEP)=0.037 mmol/L, K_(m)(ADP)=0.05mmol/L, which was the same as that from mature leaves (Fig. 1). Some metabolites, such as Asp, Asn, nalate, which activated PK_(c)from mature leaves, had no effect on PK_(c)from young leaves; #alpha#-ketoglutarate, which activated PK_(c) from mature leaves, had inhibitory effect on PK_(c)from young leaves. Fumarate, which did not affect PK_(c)activity in mature leaves, inhibited PK_(c)in young leaves. ATP slightly inhibited PK_(c)in young leaves and had no effect at its physiological concentration, whereas it inhibited PK_(c)potently in mature leaves.

Key words: Young leaf Passiflora edulis;Cytosolic pyruvate kinase;Metabolites;Ca~(2+)

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Citing This Article：

Guo Zhenfei, Li Mingqi. Regulatory properties of cytosolic pyruvate kinase from young leaves of Passiflora edulis. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).