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Construction of microfluorometer and its application for measuring intracellular free calcium

Shen Maoxing, Zhang Jian, Zhao Yuling, Zhang Xiongde, Zhu Peihong, Yang Liping

Shanghai Institute of Physiology, Chinese Academy of Sciences .Shanghai； China(200062)

Abstract

A microfuluorometer used for measuring intracellular free calcium has been constructed.This system wa based on an XSJ-2 epifluorescence microscope with the following modification. First, the AC mercury lamp was replaced bby a DC xenon lamp to provide a stable light source.Second, anexcitation filter wheel incorporatingtwo interference filters was inserted in the lighgt path allowing the bilogical sample to be excited alternately by lights of two different wavelength.

Key words: Microfluorometer;Intracellular free calcium

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Citing This Article：

Shen Maoxing, Zhang Jian, Zhao Yuling, Zhang Xiongde, Zhu Peihong, Yang Liping. Construction of microfluorometer and its application for measuring intracellular free calcium. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).