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Variation in extracellular/intracellular Ca~(2+) does not affect tyrosine (Tyr) suppressive effect on hCG-induced progesterone production by rat corpus luteal cells in vitro

Tian Shujun, Wang Qifa, Cheng Zhiping

The Reproductive Endocrinology Laboratory, Harbin Medical University. Harbin, Heilongjiang

Abstract

Ovaries of immature female rats primed with PMSG-hCG were digested with collagenase-DNAase solution to obtain the corpus luteal cell suspensions. After pre-incubation for 1h, luteal cell suspensions were then incubated with different factors for 2h, The progesterone contents in the incubates were measured by RIA. It was demonostrated that high Ca~(2+)/high K~(+)/A23187 significantly enhanced both the basal or hCG-induced progesterone production by rat luteal cells. To the contrast, decreased Ca~(2+) concentration in medium/EGTA/verapamil had inhibitory effect on progesterone production in the presence of hCG.Tyr had suppressive effect on hCG-induced progesterone production. but not in the presence of high Ca~(2+)/high K~(+)/A23187. It suggested that progesterone production by luteal cells of rat is influenced by concentration. Yet, variation in extra/intra-cellular Ca~(2+) does not affect Tyr suppressive effect on hCG-induced progesterone production.

Key words: Calcium;Tyrosine;Progesterone;Corpus luteum cells

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Citing This Article：

Tian Shujun, Wang Qifa, Cheng Zhiping. Variation in extracellular/intracellular Ca~(2+) does not affect tyrosine (Tyr) suppressive effect on hCG-induced progesterone production by rat corpus luteal cells in vitro. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).