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Effect of ATP on cytosolic free calcium in single porcine pulmonary artery endothelial cell in primary culture

Hu Qinghua, Wang Dixun, Zhang Yu, Wang Jinxi, Chen Rongsan

Department of Pathophysiology, Tongji Medical University. Wuhan,Hubei；China(210009)

Abstract

Using a dual-wavelength excitation fluorescence spectrophotometer, it was found that ATP evoked a rapid rise in [Ca~(2+)]i followed by a slightly maintained increase, Removal of extracellar Ca~(2+)abolished the maintained increase without obvious effect on the initial transient. These results suggest that ATP stimulates both calcium release from intracellular calcium pool(s) and calcium influx across the plasma membrane.

Key words: Cytosolic free;Calcium endothelial;Cell ATP

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Citing This Article：

Hu Qinghua, Wang Dixun, Zhang Yu, Wang Jinxi, Chen Rongsan. Effect of ATP on cytosolic free calcium in single porcine pulmonary artery endothelial cell in primary culture. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).