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Semiquantitative detection and application about the expression of nitric oxide synthase gene

Zhang Chenhui, Li Qianhong, Zhang Jifeng, Zhou Hong, Zhang Xinbo, Tang Jian

Cardiovascular Basic Institute, Beijing Medical University. Beijing；China(100083)

Abstract

Using the Reverse Transcriotion (RT)-Polymerase Chain Reaction(PCR) method of Martin (1993) for semiquantitation determinating of NOS gene, it was found that NOS mRNA is not only existed in brain, but also distributed extensively in heart, kidney, lung and liver. Among of them, NOS mRNA levels were highest in the brain, followed in descending order by kidney, heart. In addition to endothelial cell, NOS gene was also highly expressed in smooth muscle cells, suggesting that they may be an important site of NOS in organism. Furthermore, NOS mRNA levels were found to decrease significately in brain, kidney, liver and smooth muscle cell in spontaneous hypertensive rat.

Key words: Nitric oxide synthase;Gene expression;Spontaneous hypertensive rat; Semiquantitative polymerase chain reaction

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Citing This Article：

Zhang Chenhui, Li Qianhong, Zhang Jifeng, Zhou Hong, Zhang Xinbo, Tang Jian. Semiquantitative detection and application about the expression of nitric oxide synthase gene. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).