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The potentiation effect of haloperidol on the binding of etorphine to brain membranes in acupuncture analgesia

Abstract

[~(3)H]-etorphine was used in receptor binding studies on membrane preparation from rabbit brain. Scatchard analysis revealed that rabbit brain had two different affinity binding sites. In the high affinity site, the dissociation constant(K_(d1)) of the control group was 2.57±0.33 nmol/L. When analgesia was induced by haloperidol or acupuncture, the K_(d1) values decreased respectively (P<0.05) to 1.44±0.03 nmol/L and 1.53±0.05 nmol/L. These K_(d1) values further decreased to 1.25±0.03nmol/L when acupuncture analgesia was potentiated by combined action of acupuncture and iv. haloperidol(P<0.01). In case of autoradiographic analysis of brain slices showed that the density of opioid receptors in many brain regions, such as N. Caudatus, preoptic lateral area, N. Paraventricularis, N. Centromedianus, Periaqueductal gray, showed significant increase, results indicating that the uprgulation of opioid receptors in rabbit brain may be one of the mechanisms in the potentiating action of haloperidol on acupuncture analgesia.

Key words: Acupuncture analgesia;Haloperidol;[~(3)H]-etorphine;Dopamine receptor;Opioid receptor;Receptor regulation

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Citing This Article：

. The potentiation effect of haloperidol on the binding of etorphine to brain membranes in acupuncture analgesia. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).