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Basolateral membrane mechanisms involved in ligustrazine-stimulated anion secretion in rat distal colon

Xing Ying, He Qiong, Zhu Jinxia, Chan Hsiaochang

Department of Physiology,Medical School,Zhengzhou University.Zhengzhou 450052,Henan；China

Abstract

The present study investigated the cellular mechanism underlying the effect of ligustrazine on the ion transport in rat distal colon using the short-circuit current (I_(SC)) technique. In freshly isolated colonic strips, basolateral addition of ligustrazine stimulated a rise in I_(SC), which was resistant to basolateral application of neuronal sodium channel blocker tetrodotoxin (TTX), but inhibited by 55.2% by basolateral pretreatment with prostaglandin inhibitor indomethacin. The ligustrazine-induced I_(SC) increase was inhibited by apical application of Cl~(-) channel blockers diphenylamine-2, 2^-dicarboxylic acid (DPC) and glibenclamide. Basolaterally administered bumetanide, an inhibitor of Na~(+)-K~(+)-2Cl~(-) cotransporter, inhibited ligustrazine-evoked current increases by 85.2% and basolateral exposure to Ba~(2+), a non-specific potassium channels blocker, and blocked the current by more than 90%, indicating that basolateral Na~(+)-K~(+)-2Cl~(-) cotransporter and K~(+) channels played an important role in the effect of ligustrazine. The results suggested that ligustrazine could stimulate rat distal colon Cl~(-) secretion that is mediated by basolateral Na~(+)-K~(+)-2Cl~(-) cotransporter and K~(+) channel.

Key words: Ligustrazine;Colonic mucosa;C1 secretion;Na+-K + -2C1 cotransporter;K+ channels

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Citing This Article：

Xing Ying, He Qiong, Zhu Jinxia, Chan Hsiaochang. Basolateral membrane mechanisms involved in ligustrazine-stimulated anion secretion in rat distal colon. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).