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Characteristics of apyrase (EC 3.6.1.5) on cultured bovine endocardial endothelial cells

Yi Fuxian, Sun Ping, Huang Shaoling, Liu Wenlan, Guo Zhaogui

Laboratory of Molecular Pharmacology,Hunan Medical University.Changsha 410078,Hunan

Abstract

Apyrase activity was assayed by inorganic phosphate release, which could be inhibited concentration-dependently by NaN_(3), an apyrase inhibitor. NaF (20 mmol/L), another inhibitor of apyrase, also markedly inhibited the activity. EDTA or EGTA (1 mmol/L) could also inhibit the activity completely. However, the inhibitor for Na~(+)/K~(+)-ATPase, ouabain (3 mmol/L) did not affect the enzyme activity.

Key words: Apyrase;Adenosine diphosphate;Endocardium;Sodium azide

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Citing This Article：

Yi Fuxian, Sun Ping, Huang Shaoling, Liu Wenlan, Guo Zhaogui. Characteristics of apyrase (EC 3.6.1.5) on cultured bovine endocardial endothelial cells. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).