Issue Archive

Changes in polyribosome level and protein synthesis of wheat leaves in the early stage of stripe-rust infection

Zhang Hui, Shang Hongsheng, Li Zhenqi

Department of Plant Protection, Northwestern Agricultural University. Yangling, Shaanxi

Abstract

After wheat(Triticum aestivum L.CV Lovrin 13)primary leaves inoculated with virulent race(CY29)of stripe-rust fungus (Puccinia striiformis West) and its avirulent mutant (CY29-mut3), the rate of soluble protein synthesis in vivo in incompatible host leaves was higher than uninoculated leaves 24 h after inoculation, and then declined. In compatible host leaves, however, though soluble protein synthesis was the same as uninoculated leaves during the early stage of infection, a sharp increase in the rate of synthesis of membrane bound protein occurred 96 h after inoculation. Meanwhile, analysis of ribosome in wheat primary leaves by sucrose density gradient centrifugation confirmed that there was predominant increase of free polyribosome level in incompatible host and of membrane bound polyribosome level in compatible host. It is conclued that reactions of both the resistant and susceptible host plants involve alterations in protein synthesis ability.

Key words: Wheat;Wheat stripe rust;In vivo protein synthesis;Ployribosome;Rust resistance

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Zhang Hui, Shang Hongsheng, Li Zhenqi. Changes in polyribosome level and protein synthesis of wheat leaves in the early stage of stripe-rust infection. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).