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Isolation and identification of calmodulin-binding proteins from Pinus yunnanensis pollen

Gong Ming, Yang Zhonghan, Cao Zongxun

Department of Biology, Peking University. Beijing

Abstract

Ten CaM-binding proteins were isolated from Pinus yunnanensis pollen by CaM-Sepharose 4B affinity chromatography. These proteins could inhibit CaM-activated PDE activity, which could be eliminated by adding-an excess of exogenous CaM. The assay of enzyme activity indicated that there were Ca~(2+)-and CaM-dependent ATPase activities, but no peroxidase, phytase, acid phosphatase and phospholipase D activities in CaM-binding proteins of pine pollen.

Key words: CaM-binding proteins;ATPase;Pollen;Pinus yunnanensis

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Citing This Article：

Gong Ming, Yang Zhonghan, Cao Zongxun. Isolation and identification of calmodulin-binding proteins from Pinus yunnanensis pollen. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).