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Selection of variants with high yield of oligosaccharin from Panaxginseng cultured cells

Luo Jianping, Zheng Guangzhi, Gan Fanyuan

Kunming Institute of Botany, The Chinese Academy of Sciences. Kunming,Yunnan

Abstract

Cultured cells of Panax ginseng were found to be highly sensitive to UV-irradiation, which could markedly decrease the plating efficiency of single cell cloning of P. ginseng cultured cells. After cultured cells were treated with UV-irradiation for 30 s, the plating efficiency was 21.4% of the control. Cell growth rate and oligosaccharin content of all 122 clone lines obtained from 165 clone lines through 3 times transplantation culture, chosen after 60 d of plating culture, were measured. Basing on the stability of clone lines obtained through 10 generations successive subculture, a stable clone line PGUA-08 with high yield of oligosaccharin had been selected. Moreover, the isozyme patterns of peroxidases of clone line PGUA-08 maintained almost unchanged during successive subculture. The growth rate of clone line PGUA-08 was 0.537 g DW~(-1)L~(-1)d~(-1), and was 46% higher than that of its parent, and its oligosaccharin content and yield were 17.16% DW and 2.764 g/L, respectively, 1.81 and 2.62 times that of its parent, respectively.

Key words: Panax ginseng single cell clone;Clone lines;Oligosaccharins;UV--irradiation;Peroxidase isozyme;

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Citing This Article：

Luo Jianping, Zheng Guangzhi, Gan Fanyuan. Selection of variants with high yield of oligosaccharin from Panaxginseng cultured cells. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).