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The transient gene expression of exogenous GUS genes in cauliflower hypocotyl protoplasts

Yang Zhongnan, Xu Zhihong, Bai Yongyan, Wei Zhiming

National Key Lab of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai

Abstract

The transient expression of GUS gene mediated by 20% PEG was higher than that mediated by 13% PEG. However, 13% PEG was more benificial than 20% PEG for high protoplast survival; Heat shock improved the expression, but it was harmful to protoplasts; The state of protoplasts affected the expression greatly. The Gus gene expression in protoplasts isolated from 5-day-old hypocotyls was much stronger than that from 8-day-old hypocotyls; Plasmid pKI-WI101 gave a much stronger transient expression than plasmid pBI221.

Key words: Cauliflower;Hypocotyl;Protoplast;PEG;Transient expression

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Citing This Article：

Yang Zhongnan, Xu Zhihong, Bai Yongyan, Wei Zhiming. The transient gene expression of exogenous GUS genes in cauliflower hypocotyl protoplasts. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).