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Study on the action site of oligomycin in chloroplast

Wei Jin

Shanghai Inst. Plant Physiol., Acad. Sin.

Abstract

The inhibitory effect of oligomycin on the activity of membrane Mg super(2+)-ATpase from CF sub(1) on membrane and Ca super(2+)-ATPase of soluble CF sub(1). Illumination induced some conformational changes of the enzyme, which caused the exposure of GAMMA-subunit and easy digestion by trypsin. Both ATPases with exposed GAMMA-subunit were highly sensitive to oligomycin, the action site of which was shown to be on the GAMMA-subunit of CF sub(1). The Mg super(2+)-ATPase activated by TPCK-trypsin of chloroplast treated with NEM, a GAMMA-subunit modifying agent was not affected by oligomycin.

Key words: Oligomycin;NEM

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Citing This Article：

Wei Jin. Study on the action site of oligomycin in chloroplast. Acta Physiol Sin 1991; 43 (2): (in Chinese with English abstract).