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Observations on the growth development of PLBs in Cymbidium with scanning electron microscope

Wang Xiong, Wu Dunsu

Shanghai Inst. Plant Physiol., Acad. Sin.

Abstract

The protocorm-like bodies(PLBs) of Cymbidium ensifolium were cultured in two 1/2 MS sub(0) media with or without 3% sugar. Many meristematic regions at different development stages distributed on the surfaces of PLB sub(s). The cells in meristematic regions developed into rosette PLB sub(s) and thus multiplied. Continuous illumination enhanced the multiplication of meristematic regions, while continuous darkness was unfavorable to the development of meristematic regions. When the PLB sub(s) were cultured in the medium without sugar, the cells in meristematic regions elongated and developed into tube-like tissues and lost the capacity to multiply. Leaf primordia were found on the tips of PLB sub(s), which differentiated into cells resembling the guard cells of stomata.

Key words: Cymbidium;PLB;Ontogenesin;

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Citing This Article：

Wang Xiong, Wu Dunsu. Observations on the growth development of PLBs in Cymbidium with scanning electron microscope. Acta Physiol Sin 1991; 43 (2): (in Chinese with English abstract).