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产前束缚应激对子代大鼠海马神经干细胞增殖及巢蛋白表达的影响

陈蕊, 张玉荣, 蔡东阁, 苏倩

西安医学院第一附属医院妇科，西安 710077；西安医学院第一附属医院药剂科，西安 710077；西安交通大学第二附属医院妇产科，西安 710004；西安交通大学第一附属医院儿科，西安 710061

摘要

为了观察产前束缚应激对子代大鼠空间学习记忆能力、海马神经干细胞增殖及巢蛋白表达的影响，将体重240~260 g的Sprague-Dawley雌性母鼠12只随机分成2组，对照组于孕期不做任何处理，束缚应激组于孕14~20 d时给予束缚应激，3次/天，45 min/次。取1月龄子代大鼠进行实验研究。Morris水迷宫定位航行实验结果显示，应激组子代与对照组相比，到达平台的潜伏期延长(P < 0.05)，而在空间探索实验中，应激组子代在原平台象限停留时间与对照组相比无显著差异。免疫组织化学结果显示，应激组雌性子代海马巢蛋白(nestin)和BrdU阳性细胞表达均较对照组显著增加(P < 0.05)，而雄性子代海马nestin和BrdU阳性细胞表达与对照组相比无显著性差异(P > 0.05)。以上结果提示，产前束缚应激可引起雌性子代大鼠海马神经干细胞数量增加以及增殖能力增强，可能与机体对产前应激所致脑损伤的代偿性反应相关。

关键词： 产前束缚应激; 海马 ; Morris水迷宫 ; 神经干细胞 ; 增殖

分类号：R363

Effects of prenatal restraint stress on neural stem cell proliferation and nestin protein expression in the hippocampus of offspring rats

Chen Rui, ZHANG Yu-Rong, CAI Dong-Ge, SU Qian

Department of Gynecology, The First Affiliated Hospital of Xi’an Medical University, Xi’an 710077, China； Department of Pharmacy, The First Affiliated Hospital of Xi’an Medical University, Xi’an 710077, China； Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China； Department of Pediatrics, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China

Abstract

To investigate the effect of prenatal stress on neural stem cell proliferation and nestin protein expression in the hippocampus of rat offspring, Sprague-Dawley female rats weighing 240–260 g were included and randomly divided into control and restraint stress groups. Rats in the control group were given no interventions. Rats in the restraint stress group were given restraint stress three times daily for 45 min during gestational days 14–20. One-month-old offspring rats were used for further experiment. The results of Morris water maze showed that the escape latency to reach the platform in offspring of restraint stress group was longer than that in control group (P < 0.05). There was no significant difference of the staying-time in quadrant of the primary platform in spatial probe test. The expressions of nestin- and BrdU-positive cells in the hippocampus of female offspring in restraint stress group were significantly higher than those in the control group (P < 0.05), but there was no significant difference in male offspring (P > 0.05). These results suggest that prenatal restraint stress can increase the number and proliferative capability of the neural stem cells in female offspring. It may be a primary stage of the cascade reaction of the body to the brain damage caused by prenatal restraint stress.

Key words: prenatal restraint stress; hippocampus ; Morris water maze ; neural stem cell ; proliferation

收稿日期：2017-01-06　　录用日期：2017-05-08

通讯作者：张玉荣　　E-mail: zyr876281137@126.com

引用本文：

陈蕊, 张玉荣, 蔡东阁, 苏倩. 产前束缚应激对子代大鼠海马神经干细胞增殖及巢蛋白表达的影响[J]. 生理学报 2017; 69 (4): 405-412.

Chen Rui, ZHANG Yu-Rong, CAI Dong-Ge, SU Qian. Effects of prenatal restraint stress on neural stem cell proliferation and nestin protein expression in the hippocampus of offspring rats. Acta Physiol Sin 2017; 69 (4): 405-412 (in Chinese with English abstract).