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心-肺联合灌流法分离兔肺静脉肌细胞的方法

高琳琳, 张苗苗, 张亮品, 杨殊琳, 姚可珺, 宋元龙^*

华中科技大学同济医学院生理学系，武汉 430030

摘要

肺静脉中存在的心肌样细胞在阵发性房颤的发生中发挥了极重要作用。 因而研究这些心肌样细胞的生理特性有很重要的现实意义。由于缺乏有效地分离这些肌细胞的方法，使相应的科学研究工作受到很大限制。本文介绍了一种创新性的分离肺静脉肌细胞的方法，该方法的关键点是联合经主动脉逆行心脏灌注与经肺动脉的顺行肺静脉灌流，最大程度地确保了肺静脉肌细胞微环境的有效灌注。通过本方法获取的肺静脉肌细胞的数量与活性均明显高于传统的经心脏灌流消化的方法。

关键词： 肺静脉; 房颤 ; 肌细胞分离

分类号：R337.5;Q46

A rabbit pulmonary vein myocyte isolation method based on simultaneous heart and pulmonary vein perfusion

GAO Lin-Lin, ZHANG Miao-Miao, ZHANG Liang-Pin, YANG Shu-Lin, YAO Ke-Jun, SONG Yuan-Long^*

Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Abstract

Myocytes in the pulmonary veins (PV) play a pivotal role in the development of paroxysmal atrial fibrillation (AF). It is therefore important to understand physiological characteristics of these cells. Studies on these cells are, however, markedly impeded by the fact that single PV myocytes are very difficult to obtain due to lack of effective isolation methods. In this study, we described a novel PV myocyte isolation method. The key aspect of this method is to establish a combination of retrograde heart perfusion (via the aorta) and anterograde PV perfusion (via the pulmonary artery). With this simultaneous perfusion method, a better perfusion of the PV myocytes can be obtained. As results, the output and viability of single myocytes isolated by simultaneous heart and PV perfusion method were increased compared with those in conventional retrograde heart perfusion method.

Key words: pulmonary vein; atrial fibrillation ; myocyte isolatio

收稿日期：2015-08-18　　录用日期：2015-11-06

通讯作者：宋元龙　　E-mail: songyuanlong@hotmail.com

引用本文：

高琳琳, 张苗苗, 张亮品, 杨殊琳, 姚可珺, 宋元龙. 心-肺联合灌流法分离兔肺静脉肌细胞的方法[J]. 生理学报 2016; 68 (1): 50-56.

GAO Lin-Lin, ZHANG Miao-Miao, ZHANG Liang-Pin, YANG Shu-Lin, YAO Ke-Jun, SONG Yuan-Long. A rabbit pulmonary vein myocyte isolation method based on simultaneous heart and pulmonary vein perfusion. Acta Physiol Sin 2016; 68 (1): 50-56