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雷氏大疣蛛毒液对人肝癌HepG2细胞{sl p21}基因表达的影响

高莉, 沈金宝, 孙捷, 单保恩

河北师范大学生命科学学院.河北,石家庄 050016；河北师范大学汇华学院.河北,石家庄 050091；河北医科大学科技总公司.河北,石家庄 050017；河北医科大学第四医院科研中心.河北,石家庄 050011

摘要

该文研究了雷氏大疣蛛毒液对人肝癌细胞株HepG2增殖抑制作用及其分子机制。采用XTT法观察到雷氏大疣蛛毒液剂量依赖抑制HepG2细胞增殖；流式细胞仪检测发现，经过雷氏大疣蛛毒液作用的HepG2细胞周期发生明显的选择性改变；RT--PCR方法检测到{sl p21}基因表达增强；Western blot检测发现，p21蛋白表达增加。结果提示，雷氏大疣蛛毒液抑制人肝癌细胞HepG2增殖的可能机制之一是使{sl p21}基因和蛋白表达增加，G_(2)/M细胞周期被阻滞，从而诱导细胞凋亡。

关键词： 雷氏大疣蛛毒液; HepG2细胞; 细胞周期; p21基因; p21蛋白

Effect of the venom of the spider {sl Macrothele raveni} on the expression of {sl p21} gene in HepG2 cells

Gao Li, Shen Jinbao, Sun Jie, Shan Baoen

College of Life Science, Hebei Normal University.Shijiazhuang 050016,Hebei；China；Science and Technology Company, Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

This paper focuses on the effect of the venom of the spider Macrothele raveni on the proliferation of human hepatocelluar carcinoma cell line HepG2 and the related molecular mechanism. XTT test showed that the proliferation of HepG2 cells in vitro was inhibited by the spider venom (P＜0.05) in a concentration-dependent manner. By using flow cytometry, it was found that the spider venom caused selective G_(2)/M cell cycle arrest in HepG2 cells. RT-PCR and Western blot indicated the expressions of p21 mRNA and protein in HepG2 cells were obviously up-regulated by the spider venom. The venom of the spider Macrothele raveni inhibited the proliferation of HepG2 cells. These results suggest that the possible mechanism of the spider venom is to activate the expressions of p21 gene and protein and to cause selective cell cycle arrest at G_(2)/M phase, leading to HepG2 cell apoptosis.

Key words: the venom of the spider Macrothele raveni;HepG2 cells;Cell cycle;p21 gene;p21 protein

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引用本文：

高莉, 沈金宝, 孙捷, 单保恩. 雷氏大疣蛛毒液对人肝癌HepG2细胞{sl p21}基因表达的影响[J]. 生理学报 2007; 59 (1): 58-62.

Gao Li, Shen Jinbao, Sun Jie, Shan Baoen. Effect of the venom of the spider {sl Macrothele raveni} on the expression of {sl p21} gene in HepG2 cells. Acta Physiol Sin 2007; 59 (1): 58-62 (in Chinese with English abstract).