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长时间给予高选择性δ阿片受体激动剂抑制δ受体mRNA表达

王韵, 王晓民, 韩济生

北京医科大学神经科学研究中心. 北京 100083

摘要

作者采用反转录-聚合酶键反应(RT-PCR)方法，观察了δ阿片受体肽类激动剂[D-Pen~(2.5)]enkephalin(DPDPE)及非肽类激动利BW373U86对δ受体mRNA表达的影响，并比较了两者作用的差异性。结果如下：(1)DPDPE作用24及48h可使δ受体mRNA表达水平显著降低，而BW373U86只在24h有显著抑制作用;(2)DPDPE在10~(-6)mol／L时即可使δ受体的mRNA表达水平显著降低;而BW73U86在10~(-5)mol／L时才有效。可见长时间(24h)给予肽类及非肽类δ阿片激动剂均可显著抑制δ受体的mRNA表达；DPDPE作用较强，作用时程较长。

关键词： [D-Pen~（2.5）]enkephalin; BW373U86; δ阿片受体; mRNA; 下调; 反转录-聚合酶链反应

Inhibition of the expression of #delta# opioid receptor mRNA BY Long-term exposure of NG-108-15 cell line to #delta# opioid agonists

Wang Yun, Wang Xiaomin, Han Jisheng

Neuroscience Research Center，Beijing Medical University. Beijing 100083,China

Abstract

We used the method of reverse transeription-polymerase chain reaction (RT-PCR) to observe the effects of long-term adrinistration of peptide a opioid receptor agonist DPDPE and non-peptide #delta# agonist BW 73U86 on the mRNA expression of #delta# receptor and the possible difference between the two agonists.The results showed: (1) the level of #delta# receptor mRNA decreased significantly 24-48 h after administration of DPDPE; the effect of BW373U86 lasted only 24 h; and (2) DPDPE produced a signiticant decrease of δ-receptor mRNA at 10~(-6)mol/L, Whereas BW373U86 was effective only at 10~(-5)mol/L,These results suggest that long-term administration of both peptide #delta# opioid receptor agonist (24, 48 h) and non-peptide #delta# opioid agonist (24 h) significantly decreased mRNA expression of δ opioid receptor and the effect of peptide agonist DPDPE seems to be stronger and last longer than that of non-peptide agonist BW373U86.

Key words: D-Pen~（2 5）] enkephalin;BW373U86;#delta# opioid receptor;mRNA;Down-regulation reverse transcription-polyrnerase chain leaction;

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引用本文：

王韵, 王晓民, 韩济生. 长时间给予高选择性δ阿片受体激动剂抑制δ受体mRNA表达[J]. 生理学报 1998; 50 (2): .

Wang Yun, Wang Xiaomin, Han Jisheng. Inhibition of the expression of #delta# opioid receptor mRNA BY Long-term exposure of NG-108-15 cell line to #delta# opioid agonists. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).