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强啡肽A和CCK-8对大鼠脊髓突触小体摄取~(45)Ca的影响

王晓京, 王峻峰, 韩济生

北京医科大学生理教研室. 北京

摘要

为了探讨血管紧张素Ⅱ(AⅡ)和八肽胆囊收缩素(CCK-8)这两种肽的抗阿片作用机理,本实验中观察了三种阿片类物质(吗啡、强啡肽和DPDPE)和两种抗阿片物质(AⅡ和 CCK-8)对大鼠脊髓突触小体摄取~(45) Ca 的影响。结果表明:(1)在脊髓腹柱突触小体上,10nmol/L-1μmol/L 的吗啡、强啡肽 A(Dyn A)和 DPDPE 对~(45)Ca 摄取均有较弱的抑制作用;(2)CCK-8在浓度高达lμmol/L 时对~(45)Ca 摄取有较弱的抑制作用;(3)AⅡ在浓度高达lμmol/L时也不影响腹柱突触小体摄取~(45)Ca。

关键词： (45)~Ca 摄取; 脊髓背柱; 强啡肽A; 胆囊收缩素-8; 血管紧张素Ⅰ

Effects of dynorphin a and CCK-8 on synaptosomal ~(45)Ca uptake of the rat spinal cord

Wang Xiaojing, Wang Juenfeng, Han Jisheng

Department of Physiology, Beijing Medical University. Beijing, China

Abstract

Cholecystokinin octapeptide (CCK-8) and angiotensin Ⅱ (A Ⅱ) have been shown
in behavioral studies being endogenous antiopioid substrates (AOS). To assess their antiopioid mechanism at postreceptor level, we observed the effects of the three opioids and the two AOS on ~(45)Ca uptake of the rat spinal synaptosomal preparations. Morphine, Dyn A and DPDPE at concentrations of 10 nmol/L to 1#mu#mol/L,produced a mild suppression of 45Ca uptake of synaptosomal preparations from ventral spinal cord. CCK-8 showed a mild suppression only at a concentration of 1#mu#mol/L. In synaptosomes prepared from dorsal spinal cord, Dyn A but not morphine or DPDPE, produced a strong inhibition of ~(45)Ca uptake which was blocked by nor-BNI,a #kappa# receptor antagonist, at 1#mu#mol/L. While CCK-8 (10 nmol/L to 1#mu#mol/L) also suppressed ~(45)Ca uptake, it could antagonize the suppressive effect induced by Dyn A. In contrast to CCK-8, AⅡ(10 nmol/L to 1#mu#mol/L) influenced neither on synaptosomal ~(45)Ca uptake, nor on Dyn A suppression of ~(45)Ca uptake.

Key words: ~(45)Ca uptake;Dorsal spinal cord;Dynorphia A;CCK-8;Angiotensin Ⅱ

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引用本文：

王晓京, 王峻峰, 韩济生. 强啡肽A和CCK-8对大鼠脊髓突触小体摄取~(45)Ca的影响[J]. 生理学报 1990; 42 (3): .

Wang Xiaojing, Wang Juenfeng, Han Jisheng. Effects of dynorphin a and CCK-8 on synaptosomal ~(45)Ca uptake of the rat spinal cord. Acta Physiol Sin 1990; 42 (3): (in Chinese with English abstract).