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成年鼠缺血性脑损伤诱导nestin的表达

刘鹏翀, 陆世铎, 黄娅林, 孙凤艳

复旦大学上海医学院.上海(200032)

摘要

应用免疫组化和免疫荧光双标技术结合激光共聚焦扫描显微镜，观察缺血性脑损伤后脑内nestin的表达及其细胞类型。实验观察结果为，再灌后1天，在缺血中心区可见nestin阳性突起；再灌后3天和1周时，除缺血中心区外，周I、II、III区均有nestin大量表达；而2周时仅限于周边I区大量表达。激光共聚焦扫描显微镜观察到，再灌后3天，周边I区nestin阳性突起主要与GFAP共存；2周时，nestin阳性突起变粗，变长，并与NSE的共存明显增多。上述研究结果提示，脑缺血可诱导大鼠脑缺血区域表达nestin，该表达可能与神经细胞的修复有关。

关键词： 脑缺血; 荧光双标; 激光共聚焦扫描显微镜; 巢蛋白(nestin)

The expression of nestin in ischemia-injured brain of adult rat

Liu Pengchong, Lu Shiduo, Huang Yalin, Sun Fengyan

National key laboratory of medical neurobiology, Shanghai Medical College, Fudan University.Shanghai(200032)

Abstract

Immunohistochemistry and double immunofluorescent labeling techniques combined with confocal laser scanning microscope analysis were used to investigate the characteristic spatial induction profile of nestin following a transient middle cerebral artery occlusion in adult rat brain. The results showed that nestin was induced in ischemic core at 1 day after reperfusion. In addition to ischemic core, the expression of nestin increased in peri-ischemic I, II and III regions at 3 days and 1 week, then it decreased and narrowed along the rim of ischemic core 2 weeks after reperfusion. Double immunofluorescent labeling showed that nestin positive cells were mostly co-stained with GFAP, a astrocyte marker, in peri-ischemic I region 3 days after reperfusion. At 2 weeks, however, nestin cells showed a long process and the cells double stained with nestin and NSE, a neuronal specific marker, increased in the ischemic brain. The results suggest that cerebral ischemic induces nestin expression in damage neurons, which might favor the neuroprotection against ischemic damage.

Key words: Cerebral ischemia;Double immunofluorescent labeling;Confocal laser scanning microscope;Nestin

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引用本文：

刘鹏翀, 陆世铎, 黄娅林, 孙凤艳. 成年鼠缺血性脑损伤诱导nestin的表达[J]. 生理学报 2002; 54 (4): .

Liu Pengchong, Lu Shiduo, Huang Yalin, Sun Fengyan. The expression of nestin in ischemia-injured brain of adult rat. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).