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Methods for the evaluation of intestinal mucosal permeability 

YAN Jing-Ting^1,2, SUN Qi¹, ZHANG Xiao-Li¹, LIU Jing-Hua³, FENG Xiao-Yan¹, ZHU Jin-Xia^1,*

¹Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China；²Department of Anesthesiology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Institute of Anesthesia and Respiratory Critical Medicine, Jinan 250000, China；³Sixth Clinical Medical College, Capital Medical University, Beijing 100029, China

Abstract

The intestinal mucosal barrier (IMB), which consists of mechanical barrier, chemical barrier, biological barrier and immune barrier, plays an important role in the maintenance of intestinal epithelium integrity and defense against invasion of bacteria, endotoxins and foreign antigens. Impaired IMB, characterized by increased intestinal mucosal permeability (IMP) and decreased transmembrane resistance (TR), has been implicated in the pathogenesis of various digestive, urinary, circulatory, neurological and metabolic dysfunctions. Electrophysiological recording of TR in the ex vivo intestinal tissues or cultured epithelial cell monolayers, or biochemical quantification of transepithelial movement of orally-administered molecular probes or specific endogenous protein molecules has frequently been used in the evaluation of IMB. In this paper, the composition and function of IMB will be summarized, with emphasis on the evaluation methods of IMP. 

Key words: intestinal mucosa; intestinal mucosal permeability; intestinal epithelial resistance; sugar molecular probe

Received: 　　Accepted:

Corresponding author: 朱进霞　　E-mail: 1959zhujinxia@163.com

DOI: 10.13294/j.aps.2022.0014

Citing This Article：

YAN Jing-Ting, SUN Qi, ZHANG Xiao-Li, LIU Jing-Hua, FENG Xiao-Yan, ZHU Jin-Xia. Methods for the evaluation of intestinal mucosal permeability . Acta Physiol Sin 2022; 74 (4): 596-608 (in Chinese with English abstract).