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Expression and regulation of XBP1 in mouse uterus during early pregnancy

GUO Si-Wei¹, YU Wen¹, WANG Xu¹, LIU Jian-Hui¹, ZHANG Xin-Yue¹, HE Cheng-Qiang¹, DING Nai-Zheng^1,*

¹Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Sciences, Shandong Normal University, Jinan 250014, China

Abstract

The transcription factor X-box binding protein-1 (XBP1) plays a key role in unfolded protein reaction. This study was aimed to investigate the expression pattern and regulation of XBP1 in the mouse uterus during early pregnancy. The methods of immunohistochemistry (IHC) and real time quantitative RT-PCR were used to test XBP1 expression in early pregnancy, artificial decidualization, oestrous cycle and hormone-regulated mouse models. The results showed that XBP1 was spatiotemporally expressed in mouse uterus during early pregnancy. The XBP1 protein was mainly detected in the luminal and glandular epithelia on days 1–4 of pregnancy, and was strongly detected in the decidual area on days 5–8 of pregnancy. Similarly, XBP1 expression was also mainly expressed in decidual cells following artificial decidualization. During the oestrous cycle, Xbp1, Xbp1u, and Xbp1s mRNA was predominantly present in proestrus. In the ovariectomized uterus, the expression of XBP1 in luminal and glandular epithelia was up-regulated after estrogen treatment. These results suggest that XBP1 is associated with embryo implantation and decidualization during early pregnancy in mice, and the expression of XBP1 in luminal and glandular epithelia may be regulated by estrogen.

Key words: Mouse; X-box binding protein-1; early pregnancy; uterus; embryo implantation

Received: 2020-04-29　　Accepted: 2020-10-20

Corresponding author: 丁乃峥　　E-mail: nzding@sdnu.edu.cn

DOI: 10.13294/j.aps.2020.0091

Citing This Article：

GUO Si-Wei, YU Wen, WANG Xu, LIU Jian-Hui, ZHANG Xin-Yue, HE Cheng-Qiang, DING Nai-Zheng. Expression and regulation of XBP1 in mouse uterus during early pregnancy. Acta Physiol Sin 2021; 73 (2): 208-216 (in Chinese with English abstract).