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[Protective effects of adiponectin against hypoxia/reoxygenation injury in neonatal rat cardiomyocytes.] [Ariticle in Chinese]

BIAN Yun-Fei, GUO Xiao-Xia, XIAO Chuan-Shi^*

Department of Cardiology, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China

Abstract

The aim of the present study is to investigate the effects of adiponectin (APN) on hypoxia/reoxygenation (H/R) injury incultured cardiomyocytes. Primary cardiomyocytes were obtained from neonatal rats by enzymatic digestion method and identified byimmunofluorescent technique. Primary cells cultured for 72 h were used in experiment and divided into 5 groups randomly: Controlgroup, H/R group, H/R+APN group, H/R+APN+adenine 9-β-D-arabinfuranoside (AraA, AMPK inhibitor) group, and H/R + AraAgroup. The cardiocyte morphology and beating rate were observed under inverted microscope. The DNA ladder was examined byagarose gel electrophoresis, and the cell apoptosis was determined by flow cytometry. Moreover, the malondialchehyche (MDA)content in myocardial cells and the superoxide dismutase (SOD) activity in the supernatant were measured using kits, the fluorescenceintensity of intracellular Ca2+ was observed by laser scanning confocal microscope, and the phosphorylation of AMPK was determinedby Western blotting. Compared with control group, H/R group showed increased apoptotic rate, oxidative stress level, intracellular Ca2+concentration and phosphorylation level of AMPK (P<0.05), while significant ameliorations in the above indices were seen inH/R+APN group. On the contrast, AraA attenuated the protective effect of APN and decreased the phosphorylation of AMPK. Theseresults suggest that adiponectin can protect cardiomyocytes from H/R-induced oxidative stress and apoptosis through AMPK pathway.

Key words: adiponectin; cardiomyocyte; hypoxia/reoxygenation; AMP-activated protein kinase; adenine 9-β-D-arabinfuranoside

Received: 2009-09-01　　Accepted: 2010-01-25

Corresponding author: 肖传实　　E-mail: ganxibaozhongxin@sina.com

Citing This Article：

BIAN Yun-Fei, GUO Xiao-Xia, XIAO Chuan-Shi. [Protective effects of adiponectin against hypoxia/reoxygenation injury in neonatal rat cardiomyocytes.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (2): 149-155 (in Chinese with English abstract).