Issue Archive

[Anti-aging effect of transplantation of mouse fetus-derived mesenchymal stem cells.] [Ariticle in Chinese]

Li Jun, ZHANG Yuan, LIU Ge-Xiu^*

Institute of Hematonosis, Medical School, Jinan University, Guangzhou 510632, China

Abstract

To determine the role of allogeneil graft of mesenchymal stem cells in mammalian longevity, mesenchymal stem cells wereisolated from BALB/c mouse uterine-incision delivery fetus by two successive density gradient centrifugations, and then were purifiedand amplified by adherent culture. Identified P1 mesenchymal stem cells were injected (i.v.) through vena caudalis into the 15-montholdfemale BALB/c mice three times. The mice were evaluated with ultrasoundcardiogram, autopsy, score of cardiac, skin, lung, kidney,colon histopathology and serum total superoxide dismutase activity, maleic dialdehyde content, glutathione peroxidase activity. Theresults showed that after transplantation, the long-term surviving stem cells were found to be located in many organ tissues with in situY chromosomal hybridization dyeing. Median life span was increased in these animals after transplantation. Skin, cardiac, lung, kidneyand colon pathology development were delayed. The retrogradation of heart function was attenuated, the increase of heart mass index(the mass of heart/the mass of the body), and serum maleic dialdehyde content, the decrease of spleen mass index (the mass of spleen/the mass of the body), serum total superoxide dismutase activity and glutathione peroxidase activity were reduced three months aftertransplantation (all P<0.05). These results support the idea that longevity can be enhanced by transplantation of mesenchymal stemcells and reinforce the hypothesis of mesenchymal stem cell as antiager.

Key words: mouse fetus; stem cell; transplantation; anti-aging

Received: 2009-10-09　　Accepted: 2009-11-28

Corresponding author: 刘革修　　E-mail: tliugx@jnu.edu.cn

Citing This Article：

Li Jun, ZHANG Yuan, LIU Ge-Xiu. [Anti-aging effect of transplantation of mouse fetus-derived mesenchymal stem cells.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (1): 79-85 (in Chinese with English abstract).