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Induction of robust senescence-associated secretory phenotype in mouse NIH-3T3 cells by mitomycin C

HUANG Wei-Xing, GUO Xiao-Xuan, PENG Zhong-Zhi, WENG Chun-Liang, HUANG Chun-Yan, SHI Ben-Yan, YANG Jie, LIAO Xiao-Xin, LI Xiao-Yi, ZHENG Hui-Ling, LIU Xin-Guang, SUN Xue-Rong

1Institute of Aging Research, Dongguan Scientific Research Center； 2The Second Clinical School； 3School of Laboratory Medicine； 4Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan 523808, China； 5Institute of Biochemistry &； Molecular Biology, Guangdong Medical University, Zhanjiang 524023, China

Abstract

Senescence-associated secretory phenotype (SASP) is often a concomitant result of cell senescence, embodied by the enhanced function of secretion. The SASP factors secreted by senescent cells include cytokines, proteases and chemokines, etc, which can exert great influence on local as well as systemic environment and participate in the process of cell senescence, immunoregulation, angiogenesis, cell proliferation and tumor invasion, etc. Relative to the abundance of SASP models in human cells, the in vitro SASP model derived from mouse cells is scarce at present. Therefore, the study aimed to establish a mouse SASP model to facilitate the research in the field. With this objective, we treated the INK4a-deficient mouse NIH-3T3 cells and the wildtype mouse embryonic fibroblasts (MEF) respectively with mitomycin C (MMC), an anticarcinoma drug which could induce DNA damage. The occurring of cell senescence was evaluated by cell morphology, β-gal staining, integration ratio of EdU and Western blot. Quantitative RT-PCR and ELISA were used to detect the expression and secretion of SASP factors, respectively. The results showed that, 8 days after the treatment of NIH-3T3 cells with MMC (1 μg/mL) for 12 h or 24 h, the cells became enlarged and the ratios of β-gal-positive (blue-stained) cells significantly increased, up to 77.4% and 90.4%, respectively. Meanwhile, the expression of P21 protein increased and the integration ratios of EdU significantly decreased (P < 0.01). Quantitative RT-PCR detection showed that the mRNA levels of several SASP genes, including IL-6, TNF-α, IL-1α and IL-1β increased evidently. ELISA detection further observed an enhanced secretion of IL-6 (P < 0.01). On the contrary, although wildtype MEF could also be induced into senescence by MMC treatment for 12 h or 24 h, embodied by the enlarged cell volume, increased ratios of β-gal-positive cells (up to 71.7% and 80.2%, respectively) and enhanced expression of P21 protein, the secretion of IL-6 displayed no significant change. Our study indicated that, although MMC could induce senescence in both mouse NIH-3T3 cells and wildtype MEF, only senescent NIH-3T3 cells displayed the canonical SASP phenomena. Current study suggested that senescent NIH-3T3 cells might be an appropriate in vitro SASP model of mouse cells.

Key words: senescence; mice ; senescence-associated secretory phenotype ; p16INK4a ; fibroblasts

Received: 2016-07-11　　Accepted: 2016-10-25

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Citing This Article：

HUANG Wei-Xing, GUO Xiao-Xuan, PENG Zhong-Zhi, WENG Chun-Liang, HUANG Chun-Yan, SHI Ben-Yan, YANG Jie, LIAO Xiao-Xin, LI Xiao-Yi, ZHENG Hui-Ling, LIU Xin-Guang, SUN Xue-Rong. Induction of robust senescence-associated secretory phenotype in mouse NIH-3T3 cells by mitomycin C. Acta Physiol Sin 2017; 69 (1): 33-40 (in Chinese with English abstract).