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Serum amyloid A promotes the inflammatory response via p38-MAPK/SR-BI pathway in THP-1 macrophages

ZHU Ming-Yan, , WANG Yan, WANG Yu, PENG Feng-Ling, OU Han-Xiao, ZHENG Xiang, SHI Jin-Feng, ZENG Gao-Feng, MO Zhong-Cheng^*

1Department of Histology and Embryology, University of South China, Hengyang 421001, China； 2Department of Cardiovascular Medicine； 3Department of Anesthesiology and 4Department of Clinical Laboratory, the Second Affiliated Hospital of University of South China, Hengyang 421001, China

Abstract

To investigate the effect and mechanism of serum amyloid A (SAA) on the expression of scavenger receptor class B type I (SR-BI) and inflammatory response in THP-1 macrophages, the human THP-1 cells were treated with SAA and p38-MAPK agonist (anisomycin) or p38-MAPK inhibitor (SB203580). Then, the expressions of SR-BI, phosphorylated p38-MAPK and inflammatory factors (MCP-1, TNF-α, IL-1β) were examined by real-time quantitative PCR, Western blotting and ELISA, respectively. The results
showed that, compared with control group, SAA increased the levels of inflammatory factors (MCP-1, TNF-α, IL-1β), down-regulated the expressions of SR-BI, and up-regulated the expression of phosphorylated p38-MAPK protein in a concentration- and time-dependent manner in THP-1 cells (P < 0.05). After treatment with SAA and p38-MAPK agonist (anisomycin) in THP-1 cells, the expression of SR-BI was down-regulated, and the levels of inflammatory factors and phosphorylated p38-MAPK protein expression were increased, compared with the group only treated by SAA (P < 0.05). In contrast, the SR-BI expression was up-regulated, whereas inflammatory factors and phosphorylated p38-MAPK protein expressions were decreased after the cells were treated with SAA and p38-MAPK inhibitor (SB203580) (P < 0.05). The results suggest that SAA-promoted inflammatory response in THP-1 macrophages
may be through the phosphorylation of p38-MAPK and inhibition of SR-BI expression.

Key words: scavenger receptor class B type I; serum amyloid protein A; p38-MAPK; atherosclerosis

Received: 2016-01-02　　Accepted: 2016-03-11

Corresponding author: 莫中成　　E-mail: zhchmo@hotmail.com

Citing This Article：

ZHU Ming-Yan, , WANG Yan, WANG Yu, PENG Feng-Ling, OU Han-Xiao, ZHENG Xiang, SHI Jin-Feng, ZENG Gao-Feng, MO Zhong-Cheng. Serum amyloid A promotes the inflammatory response via p38-MAPK/SR-BI pathway in THP-1 macrophages. Acta Physiol Sin 2016; 68 (3): 293-300 (in Chinese with English abstract).