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[Actions of endogenous hydrogen sulfide on colonic hypermotility in a rat model of chronic stress.] [Article in Chinese]

LIU Ying, LIANG Cheng-Bo, QUAN Xiao-Jing, Xia Hong, LUO He-Sheng

Department of Gastroenterology, the Affiliated Hospital of Guilin Medical College, Guilin 541001，China； Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060，China

Abstract

The present study was designed to investigate the potential role of endogenous hydrogen sulfide (H2S) in chronic stress-
induced colonic hypermotility. Male Wistar rats were submitted daily to 1 h of water avoidance stress (WAS) or sham WAS (SWAS) for
10 consecutive days. The total number of fecal pellets was counted at the end of each 1 h of WAS or SWAS session. Organ bath recordings were used to test the colonic motility. H2S production of colon was determined, and immunohistochemistry and Western blot were performed on rat colonic samples to detect the distribution and expression of H2S-producing enzymes. The results showed that i) repeated WAS increased the number of fecal pellets per hour and the area under the curve (AUC) of the spontaneous contractions of colonic strips (P < 0.05), ii) repeated WAS decreased the endogenous production of H2S and the expression of H2S-producing enzymes in the colon devoid of mucosa and submucosa (P < 0.001), iii) cystathionine-γ-lyase (CSE) was strongly expressed in the cytosols of the circular and longitudinal smooth muscle cells and the nucleus of the myenteric plexus neurons, iv) cystathionine-β-synthase (CBS) was primarily localized in the cytosols of myenteric plexus neurons and weakly localized in the epithelial cells and v) inhibitors of H2S-producing enzymes increased the contractile activity of colonic strips in the SWAS rats (P < 0.001). In conclusion, the results suggest that the colonic hypermotility induced by repeated WAS may be associated with the decreased production of endogenous H2S.

Key words: chronic stress;hypermotility;hydrogen sulfi

Received: 2014-09-10　　Accepted: 2014-10-29

Corresponding author: 罗和生　　E-mail: xhnk@163.com

Citing This Article：

LIU Ying, LIANG Cheng-Bo, QUAN Xiao-Jing, Xia Hong, LUO He-Sheng. [Actions of endogenous hydrogen sulfide on colonic hypermotility in a rat model of chronic stress.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (1): 65-73 (in Chinese with English abstract).