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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Using a nitric chide (NO)-sensitive platinum microelectrode(SNM) modified withchitosan nickel (Ⅱ) complex and Nafion (Nation-CTS (Ni)-Pt), we observed the effectof nitric oxide synthase (NOS) inhibitor 7-nitro-indazole (7-NI) and N-nitro-L-arginine(L-NNA) on penicillin-treated hippocampal slices by simultaneously recording thestimulus-evoked field potentials and nitric oxide release from CA1 pyramidal neurons. Ourresults show: (1) The linear relationship range and detection limit of SNM were 4.5 ×10-4~ 1.0 ×10-8 mol/L and 5.0 × 10-8 mol/L respectively. (2) Penicillin (PEN)could elevate NO release, the number and amplitude of stimulus-evoked field potentialsspike (SEPS) in concentration-dependent manner; (3) Both 7-NI and L-NNA depressedNO release and partly reversed the effect of penicillin. The above findings suggest thatconvulsant provoking effect of NO could be inhibited by NOS inhibitors.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).