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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Angiotensin　Ⅱ(AngⅡ)-induced apoptosis was demonstrated for the first time in cultured adult rat ventricular myocytes (ARWs) isolated by retrograde heart perfusion with Krebs-Henseleit bicarbonate (KHB) buffer containing collagenase and hyaluronidase. ARWs incubated with 10μmol/L Ang Ⅱ for 48 h showed morphological features of apoptosis (cellular shrinkage, condensation of cytoplasm) and a characteristic "ladder" of DNA bands representing integer multiples of the intermucleosomal DNA length about 180~200 hp; which became more evident with further incubation up to 72 h. With shorter incubation time (≤24 h) or at a lower AngⅡconcentration (<10μmol/L), such changes failed to occur. This effect of Ang Ⅱ could be abolished by losartan (10μmol/L), verapamil (1μmol/L) or staurosporine (10 nmol/L). The above results indicate that AngⅡ-induced apoptosis in ARVMs may be mainly mediated by AngⅡ type Ⅰ(AT1) receptors with [Cd+ ]i and protein kinase C (PKC) playing a critical role.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).