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Decrease of calcitonin gene-related peptide release from mesenteric arterial bed in diabetic rats and effect of nitric oxide

Wang Man, Sun Wei, Xing Liyu, Gong Haibin, Guo Jingxuan

Institute of Vascular Medicine，Third Hospital，Beif ng Medical University. Beijing 100083,China

Abstract

Our previous work has shown that endotoxin triggers the release of calcitonin generelated peptide (CGRP) from the mesenteric arterial bed, which is partially mediated by nitric oxide. In the present study, the changes of endotoxin-induced CGRP release from the isolated mesenteric arterial bed and the CGRP mRNA levels in dorsal root ganglia (DRG) of diabetic rats were studied in relation to the effect of nitric oxide. CGRP level in periusate and the steatly-state level of mRNA for CGar in DRG were determined by RIA and semi-quantitatively by RT-PCR. The results showed that endotoxin (1-25#mu#g/ml) accumulated in perfusate caused concentration-dependent release of CGRP, which was significantly decreased in mesenteric arterial bed of diabetic rats. As compared with age-related control, the endotoxin (10 and 25#mu#g/ml)-induced CGRP release in diabetic rats was attenuated by 27% and 40%, respectively.

Key words: Diahetes;Calcitonin gene-related peptide;Nitric oxide;Endotoxin

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Citing This Article：

Wang Man, Sun Wei, Xing Liyu, Gong Haibin, Guo Jingxuan. Decrease of calcitonin gene-related peptide release from mesenteric arterial bed in diabetic rats and effect of nitric oxide. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).