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Modulations of proliferation between in vitro pulmonary artery endothelial cells and smooth muscle cells under hypoxia

Wang Peiyong, Liu Jian, Yu Zhonghe, Xu Shumin, Wang Junyuan, Sun Bingyong

Research Laboratory of High Altitude Medicine，Department of Pathophysiolog，Third Military Medical University. Chongqing 400038,China

Abstract

Vascular edothelial cells (VECs) and vascular smooth muscle cells (VSMCs) are structurally and functionally closely connected. Their interactions may play important roles in the development of hypoxic pulmonary artery hypertension. In the present study,mitogenic regulations between cultured new bovine pulmonary arterial endothelial cells (PAECs) and pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic conditions were investigated. Cell proliferation was assessed by ~(3)H-thymidine incorporation and flow cytometry. Normoxic conditioned medium (CM) from cultured PASMCs inhibited ~(3)H-thymidine incorporation of PAECs by 58% (P<0.01), but normoxic or hypoxic CM from PAECs promoted or inhibited ~(3)H-thymidine incorporation of PASM by 60% and 27% respectively (P<0.01).

Key words: Hypoxia;Pulmonary endothelial cell;Pulmonary smooth muscle cell;Proliferation coculture;

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Citing This Article：

Wang Peiyong, Liu Jian, Yu Zhonghe, Xu Shumin, Wang Junyuan, Sun Bingyong. Modulations of proliferation between in vitro pulmonary artery endothelial cells and smooth muscle cells under hypoxia. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).