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Roles of intercellar Ca~(2+) and protein kinase C played in tissue factor pathway inhibitor and tissue factor expression in human umbilic vein endothelial cells

Han Jianzhong, Li Juncheng, Qu Shulin, He Shilin

Department of Physiology, Hunan Medical University. Changsha 410078, Hunan, China

Abstract

The purpose of the present study was to elucidate the roles that protein kinase C (PKC) and calcium played in the tissue factor (TF) synthesis and tissue factor pathway inhibitory (TFPI) release in human umbilic vein endothelial cells (HUVEC). A_(23187) was used to represent calcium ionophore and phorbol 12-myristate 13-acetate (PMA) as that of PKC activator. TF activity in the lysed HUVEC was measured using one stage clotting assay. TFPI activity in the conditioned medium of HUVEC was assessed by the two-step chromoogenic method. The results showed that the TF activities in A_(23187), PMA and A_(23187)+PMA groups were remarkably higher (P<0.01) than that in control. Among the three treated groups, the TF activities in both A_(23187) group and A_(23187)+PMA group were lower than that in the PMA group (P<0.05), but the difference between the former two groups was statically insignificant (P>0.05).

Key words: Vascular endothelial cell;Tissue factor;Tissue factor pathway inhibitor;Protein kinase C;Calcium

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Citing This Article：

Han Jianzhong, Li Juncheng, Qu Shulin, He Shilin. Roles of intercellar Ca~(2+) and protein kinase C played in tissue factor pathway inhibitor and tissue factor expression in human umbilic vein endothelial cells. Acta Physiol Sin 1997; 49 (6): (in Chinese with English abstract).