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Effects of hypoxia/reoxygenation on mitogen-activated protein kinase activity in cultured neonatal rabbit cardiac myocytes

Yu Wenjie, Yao Xinghai, Liu Xiuhua, Chen Kui, Niu Dadi, Su Jingyi, Tang Chaoshu

Institute of Cardiovascular Research, Beijing Medical University. Beijing 100083, China

Abstract

Cardiomyocytes subjected to brief episode of hypoxia possess a resistance to serious damaging effect exerted by a subsequent long-time hypoxia on these cells, which is called hypoxic preconditioning (PC). The pathway 0f intracellular signal transduction during hypoxia PC has not yet been validated. On a model of hypoxia/ reoxygenation (H/R) of cultured neonatal rabbit cardiomyocytes, the present study is taken to investigate the changes of mitogenactivated protein kinase (MAPK) and ribosomal S6 kinase (S6K) activity. It was found that intracellular total MAPK and nuclear MAPK, afer a 15-min period of reoxygenation preceded by a single 60-min period of hypoxia, were increased by 95% and 230%, respectively. Intracellulax S6K activity increased by 142% at 30 min of H/R vs the control group (P<0.01). Phosphatase 1 (PPase 1) inhibitor (ocadaic acid, OA 1#mu#mol/L) augmented the increase of MAPK and S6K activity induced hy H/R.

Key words: Cardiomyocytes;Hypoxia/reoxygenation;Mitogen-activated kinase;Ribosomal S6 kinase

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Citing This Article：

Yu Wenjie, Yao Xinghai, Liu Xiuhua, Chen Kui, Niu Dadi, Su Jingyi, Tang Chaoshu. Effects of hypoxia/reoxygenation on mitogen-activated protein kinase activity in cultured neonatal rabbit cardiac myocytes. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).