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Inhibition of thermal hyperalgesia and tactile allodynia by intrathecal administration of#gamma#--aminobutyric acid transporter--1 inhibitor NO--711 in rats with chronic constriction injury

Zhu Shanshan, Zeng Yinming, Wang Junke, Yan Rong, Nie Xin, Cao Junli

Department of Anesthesiology,the First Affiliated Hospital,China Medical University.Shenyang 110001,Liaoning；China

Abstract

The present study was undertaken to explore the role of #gamma#-aminobutyric acid transporters in the neuropathic pain. On the chronic constriction injury (CCI) rats 4 doses (5, 10, 20, 40 #mu#g in group N5, N10, N20, N40, respectively) of specific #gamma#-aminobutyric acid transporter-1 inhibitor NO-711 or normal saline (in group NS) were intrathecally administered before sciatic nerve ligation (pretreatment) or at the third day after ligation (post-treatment). The paw withdrawl latency (PWL) from a noxious thermal stimulus and paw withdrawl mechanical threshold (PWMT) of von Frey filament was used as measure of thermal hyperalgesia and tactile allodynia respectively. The results demonstrated that post-treatment of NO-711 significantly suppressed thermal hyperalgesia and allodynia in CCI rats (P<0.05, P<0.01), the inhibitory effect lasted for 2 h (N40 group) and 4 h (N20 group) respectively. NO-711 inhibited thermal hyperalgesia induced by CCI in a dose-dependent manner. Intrathecal pretreatment with different doses of NO-711 delayed the occurrence of thermal hyperalgesia, but could not delay the emergence of allodynia induced by CCI. This study indicates that #gamma#aminobutyric acid transporter inhibiwr has anti-thermal hyperalgesia and anti-tactile allodynia effects in neuropathic rats.

Key words: neuropathy;amino acid transport systems;thermal hyperalgesia;injections, spinal

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Citing This Article：

Zhu Shanshan, Zeng Yinming, Wang Junke, Yan Rong, Nie Xin, Cao Junli. Inhibition of thermal hyperalgesia and tactile allodynia by intrathecal administration of#gamma#--aminobutyric acid transporter--1 inhibitor NO--711 in rats with chronic constriction injury. Acta Physiol Sin 2005; 57 (2): (in Chinese with English abstract).