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Effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel and Kv1.5 expression in bronchial smooth muscle cells of rats

Ye Hong, Ma Wanli, Yang Mulan, Liu Shengyuan, Wang Dixun

Department of Pathophysiology pathology, Tongji Medical College, HuaZhong University of Science and Technology, Pulmonary Disease Laboratory, Ministry of Health of China. Wuhan 430030, China；Department of Respiratory medicine, Xiehe hospital, Tongji Medical College, HuaZhong University of Science and Technology. Wuhan 430030, China

Abstract

To investigate the role of potassium channels in the pathogenesis of airway hyperresponsiveness induced by cigarette smoking, the alteration in expression of large-conductance calcium-activated potassium channel (BKca) and voltage-dependent delayed rectifier potassium channel (Kv1.5) in bronchial smooth muscle cells were investigated in chronic cigarette smoking rats. Airway responsiveness was determined, hematoxylin and eosin staining, immuno-histochemistry, in-situ hybridization and western blottechniques were used. The results showed: (1) Chronic cigarette smoking down-regulated the protein synthesis and mRNA expression of BKca and Kv1.5 in bronchial and bronchiolar smooth muscles.

Key words: Cigarette smoking;Bronchial smooth muscle cells;Potassium channel

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Citing This Article：

Ye Hong, Ma Wanli, Yang Mulan, Liu Shengyuan, Wang Dixun. Effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel and Kv1.5 expression in bronchial smooth muscle cells of rats. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).