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The maintenance of cord blood CD34+ progenitor cells with plant lectin FRIL in vitro and the expression of related cell cycle modulator HTm4 and HTm4S

Xie Xiaoyan, Xie Chao, Shi Wei, Li Jin, Li Yanhua, Wang Dongmei, Bai Cixian, Chen Lin, Pei Xuetao

Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine. Beijing 100850, China

Abstract

Exivivo maintainande of human stem cells is crucial for many clinical applications. Current culture conditions provide some level support but cytokines induce most quiescent stem cells to proliferate and differentiate. Better control of primitive is needed to extend the time and range of manipulatin of such cells. A recently identified plant lectin Flt3 receptor interacting lectin (FRIL) present may a special ability to preserve primitive CB progenitors for extended periods in culture without exogenous cytokines.

Key words: Cell biology;Hematopoietic progenitor cells;Cells cycle regulation;FRIL protein;Semi-quantitative RT-PCR;HTm4 gene;HTm4S gene

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Citing This Article：

Xie Xiaoyan, Xie Chao, Shi Wei, Li Jin, Li Yanhua, Wang Dongmei, Bai Cixian, Chen Lin, Pei Xuetao. The maintenance of cord blood CD34+ progenitor cells with plant lectin FRIL in vitro and the expression of related cell cycle modulator HTm4 and HTm4S. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).